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Microscopy
JEOL 1230 TEM
Leica Confocal System
Leica Fluorescences
Zeiss 510 Meta/ConfoCor3

Cryo/Low-Temp Preparation
Leica High Pressure Freezer
FEI Vitrobot
Cressington Quick Freeze Deep Etch
Leica MM80
Leica Freeze Subsitution

Specimen Preparation
Pelco BioWave Microwave
Cressington Sputter Coater
Polaron Critical Point Dryer
Ultramicrotomes
Denton Vacuum Evaporator

Image Analysis

Input/Output

Leica EM AFS2

The EM AFS2 is capable of freeze subsitution progressive lowering of temperature (PLT) techniques along with allowing low temperature embedding and polymerization of resins. The unit includes LED illumination for easy viewing and working within the chamber.

The freeze subsitution processor EM FSP is an automatic reagent handling system which sits atop the EM AFS2. It dispenses reagents for both freeze substitution and PLT techniques. By automatically diluting reagents from 100% stock containers, it reduces reagent handling and user set up time. All the user needs to do is add their samples and reagents, press start and then return at the end of the run. Up to 20 samples can be processed automatically per run.

    Features:
  • "Deep Freeze" allows sample transfer at temperatures below -140°C
  • Mouse controlled color screen
  • USB flash drive/memory stick connectivity
  • Fume exhaust system for operator safety
  • One step preparation
  • Efficient UV polymerization with built-in LED UV lamp
  • FS and PLT
  • "Check" function guides operator for filling reagents
  • minimizes operator contact with toxic material
  • Reduces risk of specimen loss

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Biological Imaging Facility | Hogan 5-150 & 5-120
2205 Tech Drive | Evanston, IL 60208 | 847.467.0374