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Microscopy
JEOL 1230 TEM
Leica Confocal System
Leica Fluorescences
Zeiss 510 Meta/ConfoCor3

Cryo/Low-Temp Preparation
Leica High Pressure Freezer
FEI Vitrobot
Cressington Quick Freeze Deep Etch
Leica MM80
Leica Freeze Subsitution

Specimen Preparation
Pelco BioWave Microwave
Cressington Sputter Coater
Polaron Critical Point Dryer
Ultramicrotomes
Denton Vacuum Evaporator

Image Analysis

Input/Output

Leica Confocal Laser Scanning System

Our Leica Confocal Laser Scanning System offers both inverted and standard (upright) configuration microscopes, both with fluorescence filter cubes.

    Both scopes are outfitted with identical optics:
  • 10X and 20X air objectives
  • 40X, 63X and 100X oil-immersion objectives
This system can image one transmitted and three fluorescence channels simultaneously or sequentially.
    Fluorchromes are excited by three lasers:
  • Ar (457, 476, 488, 514 nm)
  • Green HeNe (543 nm)
  • Red HeNe (633 nm)
A Galvo-z stage is used for capturing high resolution z-series.

This system is driven by Leica Confocal software on a PC workstation.

FRAP, FLIP, time-lapse imaging, and lambda scanning are possible on this system as well.

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Biological Imaging Facility | Hogan 5-150 & 5-120
2205 Tech Drive | Evanston, IL 60208 | 847.467.0374